Homonuclear Gradient Selective Double-Quantum Filtered COSY Spectra

Pulse sequence: standard Bruker pulse sequence cosygpmftp, using pulsed field gradients

Pulsed Field Gradients: sine-shaped, 1 ms duration, maximum strengths=10 G/cm and 20 G/cm (double-quantum coherence selection); 
PFG treatment method: Coherence Transfer Pathways

Spin system: three ¹H nuclei at Ha=4.2 ppm, Hb=2.8 ppm, Hb'=3.4 ppm; scalar couplings: J_ab=20 Hz, J_ab'=12 Hz, J_bb'=30 Hz

                      (this spin system corresponds to cysteine, the scalar couplings were increased for illustration puproses)

Spectrometer frequency: 500 MHz (¹H)

Acquisition: 512 t1-increments with 256 complex points (t2) each

Processing details: zero filling to 512 x 512 points, apodization using 90^o shifted sinebell, TPPI method, phase correction in F1 and F2