Pulse sequence: standard Bruker pulse sequence cosygs, using pulsed field gradients

Pulsed Field Gradients: sine-shaped, 1 ms duration, maximum strength=20 G/cm; 
gradients ratio= 1:1 for N-type coherence selection, spectra (a), (c); 1:-1 for P-type coherence selection (b); no coherence selection in (d); positions of the diagonal and antidiagonal peaks, coresponding to P- and N-coherence selection, are indicated by green and blue diagonal lines 
PFG treatment methods: Coherence Transfer Pathways (a,b), Multilayer ('Salami') approach (c,d)

Spin system: three ¹H nuclei at Ha=4.2 ppm, Hb=2.8 ppm, Hb'=3.4 ppm; scalar couplings: J_ab=20 Hz, J_ab'=12 Hz, J_bb'=30 Hz

                      (this spin system corresponds to cysteine, the scalar couplings were increased for illustration puproses)

Spectrometer frequency: 500 MHz (¹H)

Acquisition: 256 t1-increments with 128 complex points (t2) each

Processing details: zero filling, apodization using 90^o shifted sinebell, magnitude mode processing