Homonuclear 1H-1H Gradient Selective COSY Spectra

      Homonuclear COSY experiment using gradient selection 
  • Pulse sequence: standard Bruker pulse sequence cosygs, using pulsed field gradients
  • Pulsed Field Gradients: sine-shaped, 1 ms duration, maximum strength=20 G/cm; 
    gradients ratio= 1:1 for N-type coherence selection, spectra (a), (c); 1:-1 for P-type coherence selection (b); no coherence selection in (d); positions of the diagonal and antidiagonal peaks, coresponding to P- and N-coherence selection, are indicated by green and blue diagonal lines 
    PFG treatment methods: Coherence Transfer Pathways (a,b), Multilayer ('Salami') approach (c,d)
  • Spin system: three 1H nuclei at Ha=4.2 ppm, Hb=2.8 ppm, Hb'=3.4 ppm; scalar couplings: Jab=20 Hz, Jab'=12 Hz, Jbb'=30 Hz

  •                       (this spin system corresponds to cysteine, the scalar couplings were increased for illustration puproses)
  • Spectrometer frequency: 500 MHz (1H)
  • Acquisition: 256 t1-increments with 128 complex points (t2) each
  • Processing details: zero filling, apodization using 90o shifted sinebell, magnitude mode processing
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